Some people were having trouble understanding why Natsuo said "Take that, Bitch" in one of the last pages of chapter 32. Some people also mentioned how Natsuo was trying to show her brother how she isn´t all that perfect as he believes, by crying in his presence (he also saw her lose the fight against Yuzuko). I believe this might also be the reason for why she said "Take that, Bitch", she perhaps thinks that that is what she should have said after having beaten her brother's bullies that one time (pic), instead of appearing like an "angel" in front of her brother's eyes and continuing to remind him how "perfect" she is. Had she perhaps shown this side of herself, he would have seen that she isn´t as perfect as he thinks she is. This I think would have lifted some pressure off of him as he wouldn´t feel as much shit anymore about himself (I think this kind of got proven as well in the last chapter, with her brother seeing how she ain´t as perfect as he thought she was, and he finally was able to move a step forward).